Coach: 
Alberto Zaccheroni
The final squad was announced on 12 May 2014.[20] The squad numbers were revealed on 25 May.[21]
| No. | Pos. | Player | DoB/Age | Caps | Club |
|---|---|---|---|---|---|
| 1 | GK | Eiji Kawashima | 20 March 1983 (aged 31) | 56 |  Standard Liège |
| 2 | DF | Atsuto Uchida | 27 March 1988 (aged 26) | 68 |  Schalke 04 |
| 3 | DF | Gōtoku Sakai | 14 February 1991 (aged 23) | 12 | VfB Stuttgart |
| 4 | MF | Keisuke Honda | 13 June 1986 (aged 27) | 56 | Milan |
| 5 | DF | Yuto Nagatomo | 12 September 1986 (aged 27) | 70 | Internazionale |
| 6 | DF | Masato Morishige | 21 May 1987 (aged 27) | 10 |  F.C. Tokyo |
| 7 | MF | Yasuhito Endō | 28 January 1980 (aged 34) | 144 | Gamba Osaka |
| 8 | MF | Hiroshi Kiyotake | 12 November 1989 (aged 24) | 25 | 1. FC Nürnberg |
| 9 | FW | Shinji Okazaki | 16 April 1986 (aged 28) | 76 | Mainz 05 |
| 10 | MF | Shinji Kagawa | 17 March 1989 (aged 25) | 57 |  Manchester United |
| 11 | FW | Yoichiro Kakitani | 3 January 1990 (aged 24) | 12 | Cerezo Osaka |
| 12 | GK | Shusaku Nishikawa | 18 June 1986 (aged 27) | 13 | Urawa Red Diamonds |
| 13 | FW | Yoshito Ōkubo | 9 June 1982 (aged 32) | 57 | Kawasaki Frontale |
| 14 | MF | Toshihiro Aoyama | 22 February 1986 (aged 28) | 6 | Sanfrecce Hiroshima |
| 15 | DF | Yasuyuki Konno | 25 January 1983 (aged 31) | 81 | Gamba Osaka |
| 16 | MF | Hotaru Yamaguchi | 6 October 1990 (aged 23) | 21 | Cerezo Osaka |
| 17 | MF | Makoto Hasebe (c) | 18 January 1984 (aged 30) | 78 | 1. FC Nürnberg |
| 18 | FW | Yuya Osako | 18 May 1990 (aged 24) | 9 | 1860 München |
| 19 | DF | Masahiko Inoha | 28 August 1983 (aged 30) | 21 | Jubilo Iwata |
| 20 | FW | Manabu Saitō | 4 April 1990 (aged 24) | 5 | Yokohama F. Marinos |
| 21 | DF | Hiroki Sakai | 12 April 1990 (aged 24) | 18 | Hannover 96 |
| 22 | DF | Maya Yoshida | 24 August 1988 (aged 25) | 41 | Southampton |
| 23 | GK | Shūichi Gonda | 3 March 1989 (aged 25) | 2 | F.C. Tokyo |